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The Uric Acid Reagent Kit (Uricase-POD Method) is an advanced in-vitro diagnostic (IVD) solution specifically designed for the precise quantitative determination of uric acid levels in human serum, plasma, and urine samples using a photometric system. Utilizing the Uricase-Peroxidase enzymatic colorimetric method, this reagent kit facilitates accurate and reliable measurement of uric acid, a critical biomarker for medical conditions such as gout, arthritis, and impaired renal function. The assay operates through a two-step enzymatic reaction: uric acid is first oxidized by uricase to produce allantoin and hydrogen peroxide, which then reacts with 4-aminophenazone (4-AP) and 2,4-Dichlorophenol sulfonate (DCPS) under the catalytic effect of peroxidase (POD). This reaction produces a red-colored quinoneimine dye, whose intensity is directly proportional to the uric acid concentration. Designed to meet the rigorous demands of diagnostic laboratories, hospitals, and clinical setups, this reagent kit ensures high specificity, sensitivity, and reproducibility, making it an invaluable tool in the diagnosis and ongoing monitoring of disorders related to abnormal uric acid levels.
Key Features
| Features | Description |
|---|---|
| Methodology | Uricase-Peroxidase (Uricase-POD) enzymatic colorimetric method |
| Sample Types | Human serum, plasma, and urine |
| Measurement Principle | Two-step oxidation reaction producing red quinoneimine compound proportional to uric acid concentration |
| Detection System | Photometric analyzer compatible |
| Applications | Quantitative determination of uric acid for diagnosis of gout, arthritis, and renal impairment |
| Specificity & Sensitivity | High specificity with minimal interference |
| Packaging | Reagent kit with all necessary reagents for multiple assays |
| Storage Conditions | Stable storage as per manufacturer guidelines for reagent integrity |
| Regulatory Status | In-vitro diagnostic (IVD) certified |
| Attributes | Description |
|---|---|
| Assay Type | Enzymatic colorimetric quantitative assay |
| Reaction Components | Uricase, Peroxidase (POD), 4-Aminophenazone (4-AP), 2,4-Dichlorophenol sulfonate (DCPS) |
| Detection Wavelength | Approx. 520 nm (quinoneimine absorbance peak) |
| Sample Volume Required | Typically low microliter volumes depending on analyzer |
| Compatibility | Suitable for automated photometric analyzers |
| Sensitivity Range | Detects low to elevated uric acid levels consistent with clinical diagnostics |
| Shelf Life | Specified by manufacturer, typically 12-24 months |
| Storage Temperature | 2-8°C (Refrigeration recommended) |
| Usage Environment | Laboratory and clinical diagnostic environments |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
Yes, the Uric Acid Reagent Kit is compatible with most standard automated photometric analyzers used in clinical laboratories.
The kit is validated for use with human serum, plasma, and urine samples, ensuring versatility in clinical diagnostics.
The Uricase-POD method uses a two-step enzymatic reaction specifically targeting uric acid, minimizing interference from other serum components thereby increasing specificity.
The assay measures the absorbance of the quinoneimine product at approximately 520 nm, which correlates directly to uric acid concentration.
The reagents should be stored refrigerated at 2-8°C, protected from light to maintain activity and stability throughout the shelf life.
Yes, the reagent kit is designed for efficient use in clinical laboratories with capabilities for multiple assays to support high sample volumes.
Brand: labgene
Country Of Origin: India
This is an in-vitro diagnostic (IVD) reagent kit for the quantitative determination of uric acid in human serum, plasma, and urine samples on a photometric system. It uses the Uricase-Peroxidase (Uricase-POD) enzymatic colorimetric method. The kit is an essential diagnostic tool for identifying conditions such as gout, arthritis, and impaired renal function.
Uric acid is a waste product primarily excreted by the kidneys. Elevated concentrations of uric acid can be indicative of conditions such as gout, arthritis, or impaired renal function. The quantitative determination of uric acid levels is a critical component of a comprehensive diagnostic workup for these disorders.
This reagent kit employs the Uricase-Peroxidase (Uricase-POD) enzymatic colorimetric method to provide accurate and reliable results. The principle of the assay is based on a two-step reaction : first, uric acid is oxidized by the enzyme uricase to form allantoine and hydrogen peroxide (H2 O2). Subsequently, the H 2 O2 reacts with 4-aminophenazone (4-AP) and 2,4-Dichlorophenol sulfonate (DCPS) under the catalytic action of peroxidase (POD) to produce a red-colored quinoneimine compound. The intensity of this red color is directly proportional to the concentration of uric acid in the sample. This enzymatic method offers high specificity and sensitivity, making it a valuable tool for hospitals, laboratories, and clinics seeking to precisely measure uric acid levels for patient diagnosis and monitoring.
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