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The Total Protein Reagent Kit (Biuret Method) is a precise in-vitro diagnostic reagent kit specifically designed for the quantitative determination of total protein concentrations in human serum and plasma samples. Employing the well-established Biuret method, this kit facilitates the formation of a violet-blue complex as proteins react with copper ions in an alkaline solution. The intensity of this color complex is measured photometrically, providing a direct correlation to total protein levels in the biological sample. This reagent kit is essential for clinical laboratories, hospitals, and diagnostic centers, offering reliable and accurate results crucial for diagnosing and managing disorders related to protein metabolism, such as liver diseases, kidney dysfunction, malabsorption syndromes, and nutritional deficiencies. With ready-to-use reagents and full compatibility with standard photometric systems, the kit ensures streamlined laboratory workflows, rapid processing, and consistent output quality, establishing it as a vital tool for healthcare professionals engaged in clinical diagnostics.
Key Features
| Features | Description |
|---|---|
| Method | Biuret method for total protein quantification |
| Sample Type | Human serum and plasma |
| Application | Quantitative determination of total protein levels |
| Reagent Format | Ready-to-use reagent kit |
| Measurement Technique | Photometric measurement of violet-blue copper-protein complex |
| Clinical Utility | Diagnosis and management of protein metabolism disorders, liver and kidney diseases |
| Compatibility | Compatible with most photometric systems used in clinical laboratories |
| Usage Environment | Hospitals, clinical & diagnostic laboratories |
| Result Accuracy | High precision and reliability for clinical diagnostics |
| Regulatory Compliance | In-vitro diagnostic (IVD) certified |
| Attributes | Description |
|---|---|
| Protein Detection Assay | Biuret reaction - copper ions reacting with peptide bonds |
| Sample Volume Required | Varies per photometric system standard operating procedure |
| Storage Condition | Store at 2-8°C, avoid freezing |
| Shelf Life | Typically 12 months from manufacturing date |
| Reaction Time | Rapid reaction suitable for automated analyzers |
| Measurement Wavelength | Approximately 540 nm (dependent on analyzer) |
| Kit Components | Biuret reagent solution, buffer, and copper salt |
| Packaging Sizes | Multiple volume options available |
| Compatibility | Suitable for manual and automated clinical chemistry analyzers |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
Yes, the kit is designed to be fully compatible with most automated photometric analyzers commonly used in clinical laboratories.
Yes, the reagent kit provides quantitative total protein measurement with the same high accuracy for both serum and plasma samples.
The reagents should be stored at refrigerated temperatures between 2-8°C and must be protected from freezing to preserve their stability and effectiveness.
Yes, the Biuret method in this kit is sensitive enough to detect decreased total protein levels, assisting in the diagnosis of hypoproteinemia related disorders.
The Biuret method specifically reacts copper ions with peptide bonds in proteins forming a violet-blue complex, ensuring specificity to total protein without interference from other serum components.
Country Of Origin: India
This in-vitro diagnostic (IVD) reagent kit is designed for the quantitative determination of Total Protein in human serum or plasma samples. Utilizing the Biuret method, the kit provides accurate and reliable results on photometric systems. It is an essential tool for diagnosing a variety of disorders related to protein metabolism, synthesis, and loss, making it suitable for clinical laboratories, hospitals, and diagnostic centers.
The Total Protein Reagent Kit is an in-vitro diagnostic product for the quantitative measurement of total protein in serum and plasma samples. The test is based on the Biuret method, where proteins in an alkaline solution react with copper ions to form a violet-blue complex. The intensity of the color produced is directly proportional to the protein concentration in the sample, which is measured photometrically.
The clinical significance of measuring total protein is substantial, as it aids in the diagnosis and management of various health conditions. Low total protein levels may indicate defective protein synthesis in the liver, protein loss from impaired kidney function, intestinal malabsorption, or nutritional deficiencies. Conversely, elevated protein levels can be associated with chronic inflammatory disorders, liver cirrhosis, and dehydration. This kit provides a reliable and precise method for clinical professionals to assess a patient's protein status, thereby supporting accurate diagnostic decisions and patient care. The ready-to-use reagents and compatibility with photometric systems ensure ease of use and efficient workflow for hospitals, clinics, and diagnostic laboratories
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