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The Creatinine Reagent Kit (Jaffe Modified Method) is a specialized in-vitro diagnostic reagent designed for the precise quantitative determination of creatinine levels in human serum, plasma, and urine samples. Leveraging the established Jaffe modified method, this kit facilitates the formation of a colored complex when creatinine reacts with picrate in an alkaline medium, allowing for accurate photometric measurement. Particularly essential for medical laboratories, hospitals, and clinical diagnostic centers, this reagent kit provides reliable data critical for evaluating renal function and diagnosing kidney diseases. The photometric system’s fixed-time kinetic method ensures rapid, reproducible results that are directly proportional to creatinine concentration. Furthermore, the kit supports assessments integral to calculating creatinine clearance, thereby aiding in the comprehensive evaluation of glomerular filtration rate (GFR). Its cost-effective nature and ease of use make it a preferred choice for high-throughput environments needing precise renal biomarker analysis.
Key Features
| Features | Description |
|---|---|
| Measurement Principle | Jaffe Modified Method using picrate reaction in alkaline medium |
| Sample Types Supported | Human serum, plasma, and urine |
| Detection Method | Fixed-time kinetic photometric measurement |
| Application | Quantitative determination of creatinine for renal function assessment |
| Accuracy | High precision and reproducibility of results |
| Turnaround Time | Rapid testing suitable for clinical environments |
| Kit Composition | Includes reagents optimized for Jaffe reaction |
| Clinical Use | Supports calculation of creatinine clearance and GFR |
| Cost Efficiency | Economical reagent kit suitable for routine testing |
| Compatibility | Designed for use with standard photometric analyzers |
| Attributes | Description |
|---|---|
| Methodology | Jaffe Modified Colorimetric Method |
| Reaction Medium | Alkaline solution with picrate |
| Sample Volume Required | Typically 10-20 µL per test (subject to analyzer specifications) |
| Test Type | In-vitro diagnostic reagent kit |
| Storage Conditions | Store at 2-8°C, avoid freezing |
| Shelf Life | 12 months from manufacturing date if stored properly |
| Reagent Stability After Opening | Stable for up to 30 days when refrigerated |
| Intended Users | Clinical laboratories and diagnostic centers |
| Calibration | Requires external calibration with standard creatinine controls |
| Measurement Range | Typically 0.1 to 20 mg/dL (dependent on analyzer settings) |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
Yes, the kit is designed to be fully compatible with standard photometric analyzers commonly used in clinical and diagnostic laboratories for automated processing.
Absolutely, the reagent kit supports quantitative analysis in human serum, plasma, and urine samples, covering the primary biological fluids used for renal function assessment.
Accuracy is optimized by following manufacturer instructions, utilizing proper sample volumes, calibrating the photometric equipment with standard creatinine controls, and maintaining reagent and sample storage conditions.
The fixed-time kinetic method enables a rapid and reproducible measurement by quantifying the rate of colored complex formation at a predetermined time point, reducing variability compared to endpoint assays.
After opening, the reagents should be refrigerated at 2-8°C and are stable for up to 30 days. Avoid repeated freeze-thaw cycles to maintain reagent integrity.
Brand: labgene
Country Of Origin: India
This is an in-vitro diagnostic (IVD) reagent kit for the quantitative determination of creatinine in human serum, plasma, and urine samples on a photometric system. The kit utilizes the Jaffe modified method, where creatinine reacts with picrate in an alkaline medium to form a colored complex. Measuring creatinine levels is crucial for estimating renal function and detecting kidney diseases.
Creatinine is a waste product filtered by the kidneys. Its concentration in blood and serum is typically stable in a healthy individual, so an increase in these levels indicates decreased excretion and, consequently, impaired kidney function. Measuring creatinine concentration is a reliable way to estimate and monitor renal function. The Creatinine Reagent Kit is based on the Jaffe modified method, a widely used and cost-effective technique. In this reaction, creatinine reacts with picrate in an alkaline solution to produce a colored complex. The rate of formation of this complex is measured photometrically and is directly proportional to the creatinine concentration in the sample. This fixed-time kinetic method allows for a rapid and accurate assessment of kidney health, making it an essential tool for hospitals, clinics, and diagnostic laboratories. The test is also used to calculate creatinine clearance, a more comprehensive measure of glomerular filtration rate (GFR).
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