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FIEO Registered Export Ready

Alkaline Phosphatase Reagent Kit (PNPP/AMP Method) for Photometric Determination

Name: Alkaline Phosphatase Reagent Kit (PNPP/AMP Method) for Photometric Determination
Brand: labgene
SKU: 8549723
Price: 3.4 USD
Availability: InStock

Sold by labgene bio-tech private limited · Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India

Price

₹ 3.40 /Numbers

In stock

Inclusive of all taxes

Minimum order: 100 Numbers

Export Snapshot

MOQ

100 Numbers

Lead time

On request

Packaging

Standard export packaging

Shelf life / longevity

Store reagents at 2-8°C

HSN Code

901890

Origin

India

Port of dispatch

On request

Export markets served

Values shown are for labgene bio-tech private limited.

MDR 2017 and ISO13485:2016

Suitable for

Importers · Distributors · Wholesalers

Mahesana Dispatch on request FIEO member

labgene bio-tech private limited

Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India

Labgene Biotech is a leading Indian manufacturer at the forefront of IVD product development. We operate an ISO 13485-certified, WHO-GMP, and USFDA-compliant facility, with all processes driven by hundreds of validated SOPs. Our R&D team has developed over 200 diagnostic tests, with 30+ currently in active use across chain laboratories, government platforms (GEM Portal), private distributors, and international export markets.

Registered address: Mahesana, India. Industry: Healthcare, Medical Supplies & Pharmaceuticals > Biotechnology.

View profile All products

Established

2019

Employees

51–100

GST

24AADCL8897L1ZQ

IEC code

AADCL8897L

Export markets

GlobalLinker Member Since

Jul 2025

Turnover

$2 MM - $5 MM

Incorporation Type

On request

Certifications & Awards

MDR 2017 and ISO13485:2016

Product details

What are the specifications of this product?

Overview

This in-vitro diagnostic (IVD) reagent kit is designed for the photometric determination of Alkaline Phosphatase (ALP) activity in serum and plasma samples. Following the recommendations of the German Society of Clinical Chemistry, the kit uses the PNPP/AMP method to provide quantitative results. It is crucial for the diagnosis of hepatobiliary and bone diseases, offering a reliable tool for clinical laboratories and hospitals.

Product Description

The Alkaline Phosphatase (ALP) Reagent Kit provides a quantitative method for measuring ALP activity in patient samples. ALP is a hydrolytic enzyme present in various tissues, with high concentrations in the liver, bone, kidney, and placenta. Pathological increases in ALP activity are commonly linked to hepatobiliary and bone diseases. In hepatobiliary disorders, elevated ALP levels can indicate bile duct obstruction due to gallstones, tumors, or inflammation. For bone diseases, increased ALP activity signifies heightened osteoblastic activity, as seen in conditions like Paget's disease, rickets (osteomalacia), bone metastases, and hyperparathyroidism.

The kit employs the PNPP/AMP method, where ALP catalyzes the hydrolysis of p-nitrophenylphosphate into p-nitrophenol and phosphate. The resulting increase in absorbance due to the formation of 4-nitrophenolate is measured photometrically and is directly proportional to the ALP activity in the sample. This precise kinetic method, with its ready-to-use reagents (after mixing R1 and R2) and clear procedure, makes it an efficient and valuable tool for hospitals and diagnostic labs to aid in the diagnosis and monitoring of a wide range of diseases.

Description provided by labgene bio-tech private limited.

Specifications

Analyte Detected	Alkaline Phosphatase (ALP)
Sample Requirements	Serum or plasma samples
Reaction Temperature	37°C (typical for enzymatic assays)
Reagent Formulation	Two-component system (R1 and R2)
Measurement Wavelength	Around 405 nm (photometric absorbance)
Assay Time	Kinetic measurement period as per protocol
Storage Condition	Store reagents at 2-8°C
Shelf Life	Refer to product packaging or datasheet
Compatibility	Suitable for automated and manual photometric analyzers
Regulatory Status	In vitro diagnostic (IVD) reagent kit

Use cases

Category	Laboratory Diagnostic Reagents > Clinical Chemistry Reagents > Enzymatic Assay Kits > Alkaline Phosphatase Assay Kits
Primary application	Wholesale, distribution, and international sourcing
Brand	labgene
Product type	Product

Logistics & handling

Lead time	On request
Packaging	As per buyer requirement
Storage condition	Store reagents at 2-8°C
Origin	India
Form	As listed by supplier

Commercials (this seller)

Minimum order	100 Numbers
Lead time	On request
Payment terms	As per negotiated commercial agreement.

Buyer questions

Frequently asked by buyers

Common questions B2B buyers ask before sourcing Alkaline Phosphatase Reagent Kit (PNPP/AMP Method) for Photometric Determination. Answers compiled from verified sellers' commercial terms and platform data.

Q: What is the principle behind the PNPP/AMP method used in this ALP reagent kit?

A: The PNPP/AMP method is based on alkaline phosphatase catalyzing the hydrolysis of p-nitrophenylphosphate (PNPP) to produce p-nitrophenol and phosphate. The formation of 4-nitrophenolate ion increases absorbance at 405 nm, which is measured photometrically to quantify ALP activity in samples.

Q: Can the kit reagents be used directly or do they require preparation?

A: The kit comes with two reagents, R1 and R2, which must be mixed before use to create the working reagent, making it ready-to-use for photometric measurement.

Q: What sample types are compatible with this ALP reagent kit?

A: This reagent kit is compatible with both serum and plasma samples for the determination of alkaline phosphatase activity.

Q: Is the kit suitable for use on automated analyzers?

A: Yes, the reagents and methodology are compatible with both manual and automated photometric analyzers commonly used in clinical laboratories.

Q: What diseases can be diagnosed or monitored using this ALP reagent kit?

A: Elevated ALP activity measured with this kit can assist in diagnosing hepatobiliary diseases such as bile duct obstruction, as well as bone diseases including Paget's disease, rickets, bone metastases, and hyperparathyroidism.

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