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The Calcium Mono Reagent Kit employing the Arsenazo III method is a specialized in vitro diagnostic tool designed for the precise quantitative determination of calcium levels in human serum, plasma, and urine samples. This kit operates on a photometric system where calcium ions react with the Arsenazo III reagent to form a stable blue-colored complex. The intensity of this coloration is directly proportional to the calcium concentration in the biological sample, enabling accurate assessment of calcium status. This diagnostic reagent is instrumental in evaluating calcium-associated disorders including hyperparathyroidism, vitamin D deficiency, neoplastic bone diseases, and renal conditions. The kit's robust and sensitive endpoint assay provides reliable results, making it an essential asset for hospitals, clinical laboratories, and diagnostic centers aiming to monitor and manage calcium metabolism abnormalities. By facilitating early and accurate detection of calcium imbalances, this reagent kit supports effective decision-making in patient care and treatment monitoring.
Key Features
| Features | Description |
|---|---|
| Methodology | Arsenazo III photometric method for calcium quantification |
| Sample Types | Human serum, plasma, and urine samples |
| Assay Type | Quantitative endpoint measurement |
| Reaction Principle | Formation of stable blue-colored complex proportional to calcium concentration |
| Application | Diagnosis and monitoring of calcium metabolism disorders |
| Accuracy | High specificity and sensitivity in calcium detection |
| Usage Setting | Suitable for hospitals, clinics, and diagnostic laboratories |
| Stability | Stable reagent formulation for consistent results |
| Test Capability | Reliable detection of hyperparathyroidism, vitamin D disorders, bone and kidney diseases |
| Compliance | Designed for in vitro diagnostic (IVD) use |
| Attributes | Description |
|---|---|
| Measurement Principle | Photometric detection via Arsenazo III-calcium complex |
| Calcium Ion Detected | Ca2+ ions |
| Colorimetric Reaction | Blue complex formation |
| Sample Volume Required | Typically 10-20 µL serum/plasma or urine per test |
| Reaction Time | Rapid reaction, typically minutes |
| Storage Conditions | Store reagent at 2-8°C, avoid freezing |
| Shelf Life | Usually 12 months from manufacture |
| Kit Components | Calcium reagent, stabilizers, and instructions/manual |
| Detection Range | Clinically relevant calcium concentration range (approx. 1.0 to 15.0 mg/dL) |
| Compatibility | Compatible with most biochemical photometric analyzers |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
The Arsenazo III method forms a stable, intense blue complex with calcium ions that is highly specific, minimizing interference from other cations and proteins, thus providing precise and reliable calcium quantification.
Yes, the Calcium Mono Reagent Kit is formulated for compatibility with most photometric biochemical analyzers commonly used in diagnostic laboratories.
The kit is validated for quantitative calcium determination in human serum, plasma, and urine samples.
Store the reagent kit at refrigerated temperatures between 2-8°C and avoid freezing to maintain stability and ensure consistent assay performance.
Yes, the detection range spans approximately 1.0 to 15.0 mg/dL, covering clinically significant low and high calcium concentrations for effective diagnosis.
The assay offers rapid reaction times, typically delivering results within a few minutes, facilitating timely clinical decision-making.
While highly specific, extremely high concentrations of certain interfering substances or abnormal sample matrices may affect readings; following manufacturer instructions minimizes these impacts.
Brand: labgene
Country Of Origin: India
This is a diagnostic in-vitro diagnostic (IVD) reagent kit for the quantitative determination of calcium in human serum, plasma, and urine samples using a photometric system. The kit utilizes the Arsenazo III method, which forms a blue-colored complex directly proportional to the calcium concentration. This test is crucial for diagnosing and monitoring disorders related to calcium metabolism, such as hyperparathyroidism, vitamin D deficiency, and various bone or kidney diseases.
Calcium is an essential mineral, with approximately 99% stored in bones. The remaining calcium in serum exists in either a free ionized form or bound to proteins like albumin. Measuring serum calcium is vital because its levels are indicators for various health conditions. Increased calcium levels may suggest hyperparathyroidism, vitamin D intoxication, multiple myeloma, or certain neoplastic bone diseases. Conversely, decreased serum calcium may be observed in hypoparathyroidism, vitamin D deficiency, and certain kidney-related conditions like nephrosis and nephritis.
This reagent kit uses the Arsenazo III method to accurately measure calcium concentration. The principle is based on the reaction of calcium ions (Ca2+) with the chromogenic reagent Arsenazo III. This reaction forms a stable, blue-colored complex, and the intensity of this color is directly proportional to the calcium concentration in the sample. This simple, reliable, and sensitive endpoint method is well-suited for use in hospitals, clinics, and diagnostic laboratories to aid in the diagnosis and management of a wide range of metabolic and endocrine disorders.
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