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The Waters WAT054275 Symmetry C18 HPLC Column is expertly engineered for high performance reversed-phase chromatography applications. Measuring 4.6 mm in inner diameter and 250 mm in length, with a particle size of 5 µm and pore size of 100 Å, this column offers exceptional separation efficiency. Constructed with high-purity spherical silica particles, the C18 chemistry ensures superior hydrophobic interactions, enabling consistent and reproducible retention of analytes. The column is endcapped and exhibits low silanol activity, which minimizes secondary interactions and peak tailing, thereby providing sharp, symmetrical peaks suitable for complex sample analysis. With a robust maximum pressure rating of 6000 psi (415 Bar), it is compatible with most high-performance liquid chromatography (HPLC) systems, supporting demanding analytical workflows. The surface area of 335 m²/g and carbon load of 19% optimize retention time and selectivity, making the Symmetry C18 column a reliable choice for pharmaceutical, environmental, and biochemical laboratories. This product supports a pH range of 2 to 8, making it versatile for a wide variety of sample chemistries and applications.
Key Features
| Features | Description |
|---|---|
| Chemistry | C18 |
| Separation Mode | Reversed Phase |
| Particle Substrate | Silica |
| Particle Size | 5 µm |
| Pore Size | 100 Å |
| Inner Diameter | 4.6 mm |
| Length | 250 mm |
| Maximum Pressure | 6000 psi (415 Bar) |
| Endcapped | Yes |
| Silanol Activity | Low |
| Particle Shape | Spherical |
| Surface Area | 335 m²/g |
| Carbon Load | 19% |
| pH Range | 2 to 8 |
| USP Classification | L1 |
| Format | Column |
| Endfitting Type | Waters |
| Attributes | Description |
|---|---|
| Brand | Symmetry |
| Product Type | Columns |
| UNSPSC Code | 41115709 |
| System Compatibility | HPLC |
| DOM Unit | UNT |
| MOQ | 1 |
| Store Name | VOLTEDGE TRADE |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
The maximum operating pressure is 6000 psi (415 Bar), suitable for high-performance HPLC systems.
Yes, it supports a pH range from 2 to 8, allowing compatibility with both acidic and moderately basic mobile phases.
Yes, the column is endcapped and has low silanol activity to reduce secondary interactions and improve peak shape.
The column has a particle size of 5 µm and a pore size of 100 Å, providing optimized resolution and analyte retention.
The carbon load is 19%, which ensures effective hydrophobic interaction and retention in reversed-phase chromatography.
Country Of Origin: India
Specifications
Chemistry
C18
Separation Mode
Reversed Phase
Particle Substrate
Silica
pH Range Min
2 pH
pH Range Max
8 pH
Maximum Pressure
6000 psi (415 Bar)
Endcapped
Yes
Silanol Activity
Low
Particle Shape
Spherical
Particle Size
5 µm
Endfitting Type
Waters
Pore Size
100 Å
Format
Column
Surface Area
335
System
HPLC
USP Classification
L1
Inner Diameter
4.6 mm
Length
250 mm
Carbon Load
19 %
UNSPSC
41115709
Brand
Symmetry
Product Type
Columns
Specifications
Chemistry
C18
Separation Mode
Reversed Phase
Particle Substrate
Silica
pH Range Min
2 pH
pH Range Max
8 pH
Maximum Pressure
6000 psi (415 Bar)
Endcapped
Yes
Silanol Activity
Low
Particle Shape
Spherical
Particle Size
5 µm
Endfitting Type
Waters
Pore Size
100 Å
Format
Column
Surface Area
335
System
HPLC
USP Classification
L1
Inner Diameter
4.6 mm
Length
250 mm
Carbon Load
19 %
UNSPSC
41115709
Brand
Symmetry
Product Type
Columns
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Bhavnagar , India
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