Molecular Markers and Plant Biotechnology (Hardback, Rukam S. Tomar, Manoj V. Parakhia, Sunil V.Patel & B.A. Golakiya)

Name: Molecular Markers and Plant Biotechnology (Hardback, Rukam S. Tomar, Manoj V. Parakhia, Sunil V.Patel & B.A. Golakiya)
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Molecular Markers and Plant Biotechnology is an essential hardback text, authored by Rukam S. Tomar, Manoj V. Parakhia, Sunil V. Patel, and B.A. Golakiya. Spanning 578 pages across 40 unique chapters, this comprehensive book details various molecular marker techniques critical for professionals and students in the field of biotechnology. It covers significant milestones in DNA history, laboratory safety protocols, and practical methods for molecular marker laboratory operations including comprehensive sections on DNA & RNA isolation, gel electrophoresis techniques, and molecular mapping. The book is structured to not only provide theoretical knowledge but also includes in-depth equipment descriptions, practical calculations, and valuable appendices including a glossary and practical guidelines, making it indispensable for both classroom and laboratory settings in plant biotechnology.

Key Features

Features	Description
Comprehensive Coverage	40 chapters encompassing a wide range of molecular marker techniques.
Practical Laboratory Information	Includes details about required equipment and protocols for laboratory work.
Safety Guidelines	Laboratory safety rules are presented at the beginning of the text.
Supportive Appendices	Includes basic calculations, principles for reagent preparation, and a glossary.
In-depth Technical Content	Detailed theoretical discussions on molecular biology and biotechnological techniques.

Attributes	Description
Authors	Rukam S. Tomar, Manoj V. Parakhia, Sunil V. Patel, B.A. Golakiya
Pages	578 pages
Format	Hardback
Publication Year	2023
ISBN	1234567890123

*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.

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What topics in molecular markers does the book cover?

The book covers a variety of topics, including milestones in DNA history, PCR techniques, different molecular marker methodologies (RAPD, RFLP, AFLP), and advanced topics such as gene transfer and genomic barcoding.

Does the book provide practical application instructions?

Yes, the book includes practical instructions, equipment descriptions, and safety guidelines to aid laboratory work in biotechnology.

What kind of calculations are included in the appendices?

The appendices offer calculations related to molecular weight, similarity index values, and considerations for preparing reagents in a molecular marker laboratory.

Is this book suitable for both students and professionals?

Absolutely, it serves as an essential resource for both students learning biotechnology concepts and professionals seeking to apply modern molecular techniques.

How is the book structured for ease of use?

The book is structured with clear chapters not only detailing theoretical aspects, but also including practical tips and a glossary for better understanding.

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Brand: nipa

Country Of Origin: India

The book Molecular Markers and Plant Biotechnology is a comprehensive collection of molecular marker-based techniques, presented in 40 chapters and accompanied by 578 pages of detailed information and figures. This essential resource is a must-have for those working in the field of biotechnology. In addition to the theoretical content, the book also includes practical equipment descriptions to supplement laboratory work. The inclusion of laboratory safety rules at the beginning of the text is a wise decision. The appendices provide additional support with basic calculations, principles for preparing reagents, and an abbreviations and glossary section that demonstrates the attention to detail in the text preparation. This indispensable book is an absolute necessity for both laboratory and classroom settings in the field of biotechnology.

1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis (PAGE). 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers (PCR Machines). 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA (RAPD). 16. Restriction Fragment Length Polymorphism (RFLP). 17. Amplified Fragment Length Polymorphism (AFLP). 18. Simple sequence repeats (Microsatellite). 19. Variable number of Tandem repeats (Minisatellite). 20. Inter Simple Sequence Repeat (ISSR). 21. Sequence Characterized Amplified Region (SCAR). 22. Cleaved Amplified Polymorphic Sequence (CAPS). 23. Single-Strand Conformation Polymorphism (SSCP). 24. Retrotransposon-based markers (S-SAP, IRAP, REMAP, RBIP, RGAS, SNP). 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization (FISH). 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.