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The Cryo 2 Double Density Gradient Kit is a meticulously formulated semen processing solution specifically designed for use in IUI, IVF, and Andrology laboratories. This kit includes 1 ml of 45% Density Gradient, 1 ml of 90% Density Gradient, and 5 ml of HTF-based Wash Media, all optimized to enhance sperm isolation and purification. The media utilizes silane-coated colloidal silica particles to create a double density gradient, facilitating efficient separation of motile sperm from debris and contaminants. The included wash media is a HEPES-modified Human Tubal Fluid (HTF) formulation, mirroring the natural environment of sperm within the fallopian tubes, enabling sperm handling without the need for CO2 incubation. This feature significantly simplifies laboratory workflow while preserving sperm viability. The double density gradient technique minimizes sperm exposure to oxidative stress by reducing handling time and limiting free oxygen radical damage. This kit directly supports improved outcomes in semen processing by delivering pure, motile spermatozoa for use in fertility treatments. Alongside this kit, complementary equipment and consumables are also available to fully equip and standardize your Andrology laboratory.
Key Features
| Features | Description |
|---|---|
| Composition | 1 ml 45% Density Gradient + 1 ml 90% Density Gradient + 5 ml HTF-based Wash Media |
| Media Type | Double Density Gradient Media Kit |
| Active Ingredients | Silane-coated colloidal silica for gradient media; HEPES-modified Human Tubal Fluid for wash media |
| CO2 Requirement | HEPES buffering allows media to be used without CO2 incubation |
| Intended Use | Semen processing for sperm isolation in IUI, IVF, and Andrology labs |
| Oxidative Stress Reduction | Reduces handling time to minimize free oxygen radical damage to sperm |
| Natural Mimicry | Wash media replicates natural environment of fallopian tubes for sperm |
| Packaging | Single-use sterile vials to maintain media integrity and reproducibility |
| Attributes | Description |
|---|---|
| Density Gradient Concentrations | 45% and 90% |
| Wash Media Volume | 5 ml |
| Buffering System | HEPES (Hepes Modified) |
| Media Base | Human Tubal Fluid (HTF) |
| Sterility | Sterile, ready-to-use |
| Shelf Life | Typically 12 months (vendor dependent) |
| Storage Conditions | Store at 2-8°C, protect from light |
| Compatibility | Compatible with standard Andrology laboratory equipment and sperm processing protocols |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
No, the Cryo 2 kit uses HEPES-modified media that eliminates the need for CO2 incubation, allowing for easier handling and processing.
Double density gradients improve sperm separation efficiency by creating two distinct layers (45% and 90%) that better isolate motile sperm from debris and contaminants, enhancing sample purity.
Yes, the wash media is an exact duplication of Human Tubal Fluid, making it naturally compatible with sperm handling and preparation protocols used in IVF and IUI procedures.
The Silane coating stabilizes the colloidal silica particles, ensuring consistent gradient formation and reducing aggregation, which results in reliable and reproducible sperm separation.
While primarily designed for sperm isolation, the purified sperm obtained using the Cryo 2 Double Density Gradient Kit can be effectively used in downstream cryopreservation procedures.
Brand: cryocell
Country Of Origin: India
Double Density Gradient Media Kit consisting of 1 x 1ml Density Gradient 45%, 1 x 1ml Density Gradient 90% & 1 x 5ml HTF based Wash Media, for Semen Processing in your IUI / IVF / ANDROLOGY laboratory.
The Media is Hepes Modified, hence does not require CO2 Incubation.
The Density Media is made using Silane coated colloidal Silica & the Semen Wash Media is the exact duplication of Human Tubal Fluid, which is naturally found in the fallopian tubes, the natural environment for the sperms.
Density Gradient is the ideal technique for processing Semen, as this technique reduces the handling time, thereby considerably reducing the chances of the damages caused by Free Oxygen Radicals
Semen Processing is a technique used in all Infertility clinics, to isolate the Sperms from the Semen & removing the contaminants.
Besides the Media we also provide the complete set of equipment & consumables required for your Andrology Laboratory
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East Delhi , India
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