Sartorius HEK ViP NB Medium is a specialized, chemically defined, serum-free, animal-component-free, and hydrolysate-free culture medium specifically optimized for suspension cultures of HEK293 and other human cell lines. Designed as a 'lean' medium, it facilitates efficient transient transfection protocols, including those utilizing Polyethylenimine (PEI), and supports viral vector production such as Adeno-associated virus (AAV) and lentivirus. Ideal for the entire gene therapy workflow, this medium enables seamless processing from cell thawing, expansion, DNA pre-complexing, to virus production without the need for medium exchange. Its formulation contains only essential nutrients without growth factors, promoting consistent and scalable performance from research to manufacturing levels. This medium is perfect for biotech and pharmaceutical companies focusing on gene therapy development and scalable viral vector bioproduction.
Key Features
| Features | Description |
|---|---|
| Medium Type | Chemically defined, serum-free, animal-component-free, hydrolysate-free |
| Optimized For | Suspension cultures of HEK293 and other human cell lines |
| Transfection Compatibility | Supports transient transfection, including with Polyethylenimine (PEI) |
| Viral Vector Production | Ideal for AAV and lentivirus viral vectors |
| Workflow Support | Single medium for all stages: thawing, expansion, pre-complexing DNA, virus production |
| Nutrient Composition | Contains essential nutrients with no added growth factors (lean medium) |
| Scalability | Supports seamless scale-up from research to large-scale manufacturing |
| Application | Gene therapy and scalable bioproduction workflows |
| Attributes | Description |
|---|---|
| Formulation | Chemically defined, serum-free, animal-component-free, hydrolysate-free |
| Cell Culture Type | Suspension |
| Supported Cell Lines | HEK293 and other human cell lines |
| Transfection Method | Transient transfection, compatible with PEI |
| Viral Vector Types | AAV, lentivirus |
| Nutrient Content | Essential nutrients only, no growth factors |
| Usage Phases | From cell thawing, expansion, DNA pre-complexing to virus production |
| Serum Content | Serum-free |
| Animal Component | Animal-component-free |
| Hydrolysate | Hydrolysate-free |
| Packaging | Refer to supplier for packaging details |
| Storage Conditions | Store according to manufacturer recommendations (typically 2-8°C, avoid freezing) |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
No, the HEK ViP NB Medium is specifically formulated as serum-free and animal-component-free. It is intended for use without serum to ensure defined and controlled culture conditions.
Sartorius HEK ViP NB Medium is optimized for suspension cultures of HEK293. While it may support adherent cells with adaptation, it is primarily designed and validated for suspension culture workflows.
Yes, the chemically defined and lean formulation allows seamless scale-up across research, pilot, and manufacturing scales without the need for medium change, supporting scalable viral vector production.
While PEI is the recommended and commonly used transfection reagent with HEK ViP NB Medium, other transient transfection reagents compatible with chemically defined systems may also be used, but validation is recommended.
No, the medium is formulated without added growth factors as a lean medium to reduce variability and facilitate regulatory compliance in gene therapy manufacturing workflows.
Country Of Origin: Germany
Sartorius HEK ViP NB is a chemically defined, animal-component-free, serum‑free, and hydrolysate-free medium optimized for suspension culture of HEK293 and other human cell lines. Specifically formulated as a “lean” transfection medium, it supports transient transfection (e.g., with PEI), viral vector production (AAV, lentivirus), and scalable gene therapy workflows. Suitable for all phases—from thawing, expansion, pre-complexing DNA to virus production—without medium exchange
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A chemically defined, serum-free medium optimized for transfection and viral production in HEK293 suspension cultures. Designed with essential nutrients and no growth factors, ideal for gene therapy and scalable bioproduction workflows. Supports all steps from cell thawing to virus production in a single medium.
Key Features
Chemically defined, animal-component-free, and hydrolysate-free formulation
Suited for AAV/lentivirus production and transient transfection (e.g., with PEI)
Essential nutrients without added growth factors for lean media performance
Enables seamless scale-up from research to manufacturing
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