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Methods in Plant Biotechnology, authored by K.R. Koundal, offers a comprehensive guide to essential techniques in recombinant DNA technology specifically tailored for the field of plant biotechnology. The book meticulously outlines various processes including the isolation and transformation of bacterial cells, detailed protocols for nucleic acid characterization, construction of both gene libraries, and advanced molecular analyses including DNA sequencing and gene mapping using RFLP and RAPD techniques. Designed for researchers and practitioners, this hardback edition not only covers theoretical foundations but also provides practical methodologies for implementing techniques crucial to plant genetic engineering. The addition of a glossary of important terms, and an appendix containing protocols for preparing common buffers and reagents further enhances its utility as a laboratory manual. With practical case studies, this book serves as both a crucial reference and a working guide for ongoing research and applications in plant biotechnology.
Key Features
| Features | Description |
|---|---|
| Author | K.R. Koundal |
| Format | Hardback |
| Topics Covered | Recombinant DNA technology, Gene isolation, Genetic transformation, Molecular analysis of transgenic plants |
| Practical Protocols | Includes protocols for RFPL and RAPD analysis, DNA sequencing, and construction of gene libraries |
| Glossary and Appendix | Includes a glossary of terms and preparation details for buffers and reagents |
| Research Application | Provides real-world examples of plant genetic engineering problems |
| Attributes | Description |
|---|---|
| ISBN | 978-XXXXXX-XXXX-X |
| Publication Year | 2023 |
| Number of Pages | 450 |
| Publisher | Nature Publications |
| Edition | First Edition |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
The book discusses various advanced techniques including gene isolation, transformation of bacterial cells, PCR methods, Southern and Northern blotting, and gene library construction.
Yes, it contains detailed practical protocols for a range of methodologies used in plant biotechnology, including RFPL and RAPD analysis.
The target audience includes researchers, students, and professionals in the fields of plant biotechnology, molecular biology, and genetic engineering.
The book includes a glossary of important terms and an appendix with preparation instructions for common buffers and reagents used in experiments.
It provides practical examples and case studies that highlight actual research challenges in the field of plant genetic engineering.
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