The Phenol-Sulfuric Acid method is recognized as one of the most reliable colorimetric assays for the quantitative determination of carbohydrates. It is extensively utilized across various fields, including the food industry, biochemistry, environmental science, and biofuel research. This assay is particularly useful for detecting both simple sugars and complex polysaccharides in a wide range of samples. The process involves the dehydration of carbohydrates using concentrated sulfuric acid, which leads to the formation of furfural derivatives. These derivatives then react with phenol, resulting in a measurable yellow-orange color proportional to the carbohydrate concentration, primarily analyzed at a wavelength of 490 nm. Our company specializes in the production and exportation of high-quality phenol and sulfuric acid, critical reagents needed for this trusted method of carbohydrate analysis. We ensure both consistency and purity in our chemicals to support your research and industrial applications effectively.
Key Features
| Features | Description |
|---|---|
| Colorimetric Assay | Quantitative determination of carbohydrates like simple sugars and polysaccharides. |
| Wide Application | Used across food industry, biochemistry, environmental science, and biofuel research. |
| Dehydration Process | Carbohydrates are dehydrated by concentrated sulfuric acid to form reactive furfural derivatives. |
| Spectrophotometric Measurement | Color intensity measured at 490 nm for accurate carbohydrate concentration. |
| High Purity Reagents | Manufactured under strict quality standards to support reliable results. |
| Attributes | Description |
|---|---|
| Method | Phenol-Sulfuric Acid Method |
| Main Reagents | Phenol solution (5% or 6% w/v in water), Concentrated sulfuric acid (H₂SO₄) |
| Measurement Wavelength | 490 nm |
| Applications | Food analysis, biochemical assays, environmental testing, biofuel research |
| End User Benefit | Reliable and reproducible results for carbohydrate quantification |
*Disclaimer: The above description has been AI-generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
This method is extensively adopted in the food industry for sugar content analysis, biochemistry for glycogen assessment, environmental science for water quality testing, and biofuel research for sugar quantification in biomass.
The assay requires a phenol solution (5% or 6% w/v in water), concentrated sulfuric acid, and a standard sugar solution like glucose or sucrose.
The dehydration reaction forms furfural derivatives that react with phenol, producing a yellow-orange color. The intensity of this color correlates directly to the carbohydrate concentration in the sample.
Color intensity is best measured spectrophotometrically at a wavelength of 490 nm for optimal accuracy.
Using high-purity reagents ensures that the results are reliable and reproducible, minimizing background interference and enhancing assay accuracy.
Country Of Origin: India
manufacturing and exporters of phenol
The Phenol-Sulfuric Acid method is a widely used colorimetric assay for the quantitative determination of carbohydrates, including simple sugars and complex polysaccharides. Here’s a breakdown of what it is and how it works:
Applications
Food industry (e.g., sugar content in beverages)
Biochemistry (e.g., analysis of glycogen or starch)
Environmental science (e.g., carbohydrate content in water samples)
Biofuel research (e.g., quantifying sugars in biomass)
manufacture and exports phenol sulfuric acid
Phenol-Sulfuric Acid Method Overview
Principle:
Carbohydrates are dehydrated by concentrated sulfuric acid to form furfural derivatives (e.g., hydroxymethylfurfural from hexoses).
These furfural derivatives react with phenol, producing a yellow-orange color.
The intensity of the color is proportional to the carbohydrate concentration and can be measured spectrophotometrically, usually at 490 nm.
🧪 Reagents:
Phenol solution (5% or 6% w/v in water)
Concentrated sulfuric acid (H₂SO₄)
Standard sugar solution (e.g., glucose or sucrose)
🧫 Protocol (simplified)
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