Experimental and Evidence based Protocols in Pharmacology provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures. Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application, DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen. This book imbibes all the basic and advanced research protocols along with their translational approaches in experiments involved in pharmacology and also provides the insight of example based drug assays to help in bioprospecting of naive molecules.
Further, this book also provide a reference book to the researchers involved in toxicological testing using OECD testing guideline to calculate the lethal dose of any compound in a better manner. This MS also suffice the different methods to calculate the median lethal dose (LD50). The important reproductive toxicity assays to ascertain the toxic potential of any xenobiotics is also incorporated in this MS. Moreover, sophisticated technique like Flow cytometry and other experimental models viz. Direct and Indirect LPS induced acute lung injury model has also well written to provide an insight of the experimentation involving laboratory animals.
Content
1 Preparation of Primary Cell Cultures from Different Tissues/Organs
Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava
2 Procedures to Evaluate Cytotoxicity in Primary Cell Culture
Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava
3 Genotoxicity Assays for Safety Evaluation of Chemicals inCell Culture
Atul Prakash, Rajesh Mandil and A.K. Srivastava
4 DNA Fragmentation Assay
Rajesh Mandil, Atul Prakash, S. P. Singh and Preeti Singh
5 Single Cell Gel Electrophoresis (SCGE/Comet assay) inPrimary Cell Culture
Atul Prakash and Rajesh Mandil
6 Micronuclei Induction Assay in Primary Cell Culture
Atul Prakash and Rajesh Mandil
7 Protocol for TUNEL Assay in Primary Cell Culture
Rajesh Mandil and Atul Prakash
8 Screening of Myometrial Relaxants in ExperimentalDysmenorrhoea an in vitro Model
Afroz Jahan, M. K. Lonare, Sanweer Khatoon and G. S. Rao
9 Direct and Indirect LPS Induced Acute Lung Injury Model
Meemansha Sharma, Anshuk Sharma, Thakur Uttam Singh
10 A Laboratory Protocol for Mesenchymal Stem Cell Isolationfrom Veterinary Important Species
Milindmitra K Lonare, Afroz Jahan, Manjinder Sharma, andSivaraman Ramanarayanan
11 Detection of Environmental Pollutants and AgrochemicalsUsing Gas Chromatography and Mass Spectrophotometry
Amit Shukla, Soumen Choudhury, Atul Prakash, Sakshi Tiwari
12 Basics of Flow Cytometric Analysis
Amit Shukla, Mukul Anand, Soumen Choudhury, Sakshi Tiwari
13 Determination of Aflatoxin Using HPLC
Shalini Vaswani, Mukul Anand
14 ICP-OES to Estimate Minerals in Biological Samples
Shalini Vaswani, Mukul Anand
15 Recording of Myometrial Reactivity Using Polyphysiograph
Soumen Choudhury, Amit Shukla, Preeti Singh and Atul Prakash
16 Estimation of Oxidative Stress in Hepatic Tissue
Amit Shukla and Sakshi Tiwari
17 Drug Metabolizing Enzymes Assay
Atul Prakash and Amit Shukla
18 Acetylcholine Esterase (AChE) Assay
Atul Prakash and Amit Shukla
19 Preparation of Hepatocyte Culture for Toxicity Testing ofXenobiotics
S.P. Singh and Atul Prakash
20 Detection of Apoptosis by DNA Gel Electrophoresis
Atul Prakash and Amit Shukla
21 Lethal Dose 50 Calculation Methods
Amit Shukla, Atul Prakash, Soumen Choudhury and Sakshi Tiwari
22 OECD Guidelines for Acute Animal Toxicity Tests
Amit Shukla and Sakshi Tiwari
23 Evaluation of Reproductive Toxicity Potential of Xenobiotics
Atul Prakash and Amit Shukla
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